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Welcome to the Braun lab !dieter_2014

We are interested in the synthetic evolution of early life forms. Our focus is to construct disequilibrium settings that foster autonomous molecular evolution. We aim to drive a Darwinian Process for example with a thermal gradient: convection drives replication while the thermal molecule trap drives the selection of long, information bearing replicates. Can we recreate the first steps of molecular evolution in the lab?

Along the way, we explore why biomolecules move in a temperature gradient, have established light driven microfluidics, build (opto)thermal molecule traps and study reaction kinetics in living cells.

Most notably, we developed an all-optical method to measure the binding affinity of biomolecules in complex biological fluids such as serum. Former PhD students Stefan Duhr and Philipp Baaske founded the startup company NanoTemper Technologies (Deutscher Innovationspreis 2012, Step Award 2012) that sell instrument and technology to quantify biomolecule interaction and enzyme activity.

Selected recent publications and prizes

simonsOur lab was able to win funding ($1.500.000 over five years) by the
Collaboration on the Origins of Life Investigator Award!
The competition was stiff: 2 out of 140 were funded.

bubbleThermophoresis in Nanoliter-Droplets Quantifies Aptamer Binding Susanne Seidel, Niklas Markwardt, Simon Lanzmich, Dieter Braun
Angewandte Chemie doi: 10.1002/anie.201402514 (2014)

pegDetection of Thermoresponsive Polymer Phase Transition in Dilute Low-Volume Format by Microscale Thermophoretic Depletion

Manuel Wolff, Dieter Braun and Michael A. Nash
Analytical Chemistry online 2014, DOI: 10.1021/ac5008283

 

electro_thermophoresisWhy charged molecules move across a temperature gradient: the role of electric fields Maren Reichl, Mario Herzog, Alexandra Götz and Dieter Braun
Physical Review Letters 112, 198101 (2014)
DOI: http://dx.doi.org/10.1103/PhysRevLett.112.198101
Synopsis in PRL, Press release.

antimoneyStatistical Mechanics of a Time-Homogeneous System of Money and Antimoney.

Matthias Schmitt, Andreas Schacker and Dieter Braun
New Journal of Physics 16, 033024 (2014) doi:10.1088/1367-2630/16/3/033024
Beitrag im Deutschlandfunk, Article on Phys.org, Telepolis

cgmpDry polymerization of 3',5'-cyclic GMP to long strands of RNA

Matthias Morasch, Christof Mast, Johannes Langer, Pierre Schilcher and Dieter Braun, ChemBioChem,
doi: 10.1002/cbic.201300773 (2014)

 

 

lebendiges_NGGÜbersichtsartikel: Lebendiges Nichtgleichgewicht

Christof Mast, Friederike M. Möller und Dieter Braun
Physik Journal 10:29 (2013) [PDF]
RadioWissen broadcast on the Origin of Life [22Min.]

proteinase3A monoclonal antibody (MCPR3-7) interfering with the activity of proteinase 3 by an allosteric mechanism.

Lisa C. Hinkofer, Susanne A. I. Seidel, Brice Korkmaz, Francisco Silva, Amber M. Hummel, Dieter Braun, Dieter E. Jenne, Ulrich Specks
Journal of Biological Chemistry doi:10.1074/jbc.M113.495770 (2013)



polymerization_trapEscalation of Polymerization in a Thermal Gradient

Christof B. Mast, Severin Schink, Ulrich Gerland and Dieter Braun
PNAS 110, 8030-8035 (2013), doi:10.1073/pnas.1303222110 [PDF]
Beitrag im Deutschlandfunk



step_award Stefan Duhr and Philipp Baaske

with their Team managed to win the 100.000 Euro STEP Award for their NanoTemper Technologies Startup. Congratulations!

labelfreeLabel-Free Microscale Thermophoresis Discriminates Sites and Affinity of Protein–Ligand Binding [PDF]

Susanne Seidel, Christoph J. Wienken, Sandra Geissler, Moran Jerabek-Willemsen, Stefan Duhr, Alwin Reiter, Dirk Trauner, Dieter Braun, Philipp Baaske, Angewandte Chemie DOI: 10.1002/anie.201204268 (2012)

ahaThermal, Autonomous Replicator Made from Transfer RNA

Hubert Krammer, Friederike M. Möller, and Dieter Braun
PRL 108, 238104 (2012). DOI:10.1103/PhysRevLett.108.238104
Links: APS Synopsis

innovation Stefan Duhr and Philipp Baaske

with their Team managed to win the Deutsche Innovationspreis for their NanoTemper Technologies Startup. Congratulations!

lippok Direct Detection of Antibody Concentration and Affinity in Human Serum Using Microscale Thermophoresis

Svenja Lippok, Susanne A. I. Seidel, Stefan Duhr, Kerstin Uhland, Hans-Peter Holthoff, Dieter Jenne and Dieter Braun
Analytical Chemistry 84, 3523–3530 (2012). DOI:10.1021/ac202923j

rnareactorEmergence of Information Transmission in a Prebiotic RNA Reactor

Benedikt Obermayer, Hubert Krammer, Dieter Braun, and Ulrich Gerland
PRL 107, 018101 (2011). DOI:10.1103/PhysRevLett.107.018101
Links: APS Viewpoint, PhysOrg

gpcrPeptide surfactants for cell-free production of functional G-protein-coupled receptors

Xiaoqiang Wang, Karolina Corina, Philipp Baaske, Christoph J. Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Dieter Braun, and Shuguang Zhang
PNAS 108, 9049-9054 (2011)

mstnatcomProtein-binding assays in biological liquids using microscale thermophoresis [PDF]

Christoph J. Wienken, Philipp Baaske, Ulrich Rothbauer, Dieter Braun and Stefan Duhr
Nature Communications 1, 100 (2010)
Links: Bionity, Research Highlight Nature Methods

replication_trapThermal Trap for DNA Replication [PDF]

Christof B. Mast and Dieter Braun
Physical Review Letters 104, 188102 (2010)
Press: Physics Today, FAZ, Nano 3Sat, Movie:Focus Online, Der Standard, Sueddeutsche, New Scientist, SciNexx

binding_aptamerOptical Thermophoresis for Quantifying the Buffer Dependence of Aptamer Binding [PDF]

Philipp Baaske, Christoph J. Wienken, Philipp Reineck, Stefan Duhr, Dieter Braun, Angewandte Chemie 49, 2238-2241 (2010)
Press: Deutschlandfunk (Radio)

imaging_kineticsHybridization Kinetics is Different Inside Cells [PDF]

Ingmar Schoen, Hubert Krammer and Dieter Braun
PNAS 106, 21649–21654 (2009)

molecular_conveyorAn Optical Conveyor for Molecules [PDF]

Franz M. Weinert and Dieter Braun
Nano Letters 9, 4264-4267 (2009)

optical_pumpingOptically driven fluid flow
along arbitrary microscale patterns
using thermoviscous expansion
[PDF]

Franz M. Weinert and Dieter Braun
Journal of Applied Physics 104, 104701 (2008)



Hydrothermal_AccumulationFrom the cover:
Extreme Accumulation of Nucleotides in Simulated Hydrothermal Pore Systems

Philipp Baaske, Franz M. Weinert, Stefan Duhr, Kono H. Lemke, Michael J. Russell, Dieter Braun
PNAS 104, 9346–9351 (2007)

[Commentary by Eugene V. Koonin, PNAS 104, 9105-9106 (2007)]

ThermophoresisFrom the cover:
Why molecules move along a temperature gradient

Stefan Duhr and Dieter Braun
PNAS 103, 19678–19682 (2006)

[Commentary by Dean Astumian, PNAS 104, 3-4 (2007)]

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